Topic note: experimental techniques

written by: Liran Carmel

last modified:

List of techniques:
item Immunoblot
  See Western blot.
item Western blot
  Also called protein immunoblot. It is used to detect specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein
Literature
Abbreviations:
Further topics:
Northern Blot
RT-PCR